Other

Part:BBa_K4501004:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-08)


AAVS1 3' homology arm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 342
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 342
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 342
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 425


Design Notes

Sequence was obtained from the Supplementary information of Seunghyeon Shin et al. 2020.


Source

Taken from: Shin S, Kim SH, Shin SW, Grav LM, Pedersen LE, Lee JS, Lee GM. Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells. ACS Synth Biol. 2020 Jun 19;9(6):1263-1269. doi: 10.1021/acssynbio.0c00097. Epub 2020 Jun 2. PMID: 32470292.

References