Primer

Part:BBa_K4496015:Design

Designed by: Larissa de Souza Kawanisi, Awana da Silva Lima, Paula Macedo Cunha, Gabriel Kimijima Itaziki   Group: iGEM22_USP-EEL-Brazil   (2022-09-20)


Primer (REV) optimized for BJ46a gene amplification in Escherichia coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

his primer was designed from the BJ46a circuit sequence optimized for Escherichia coli. For this, the last 8 amino acids were chosen and tested for hairpin.


Source

https://parts.igem.org/Part:BBa_K4496003

References