Coding

Part:BBa_K4496002:Design

Designed by: Ana Paula Contelli Xavier, Larissa de Souza Kawanisi   Group: iGEM22_USP-EEL-Brazil   (2022-09-14)


Optimized Bj46a for Pichia Pastoris


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Using bioinformatics tools, the best DNA gene sequence corresponding to the mRNA of the original part (UniProtKB/Swiss-Prot: Q9DGI0.1) was found. With this, The signal peptide was removed and the sequence was optimized for synthesis using the yeast Pichia Pastoris.

Source

https://www.ncbi.nlm.nih.gov/protein/Q9DGI0.1


References

BASTOS, Viviane A. et al. Natural Inhibitors of Snake Venom Metalloendopeptidases: history and current challenges. Toxins, v. 8, n. 9, p. 250, 2016.