Coding

Part:BBa_K4488018:Design

Designed by: Jessica Zhang   Group: iGEM22_Sydney_Australia   (2022-09-30)


fuBFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design was concerned with optimising the fluorescence. For example, a different SNP at the second site resulted in a dodgier fuBFP that did not fluoresce as brightly.


Source

Mark Somerville

References