Coding

Part:BBa_K4482016:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-10-10)


HIRMAb-NT3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 2569
    Illegal XbaI site found at 2330
    Illegal XbaI site found at 4311
    Illegal SpeI site found at 821
    Illegal SpeI site found at 2585
    Illegal PstI site found at 1913
    Illegal PstI site found at 3719
    Illegal PstI site found at 3885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 2569
    Illegal SpeI site found at 821
    Illegal SpeI site found at 2585
    Illegal PstI site found at 1913
    Illegal PstI site found at 3719
    Illegal PstI site found at 3885
    Illegal NotI site found at 4300
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 2569
    Illegal BamHI site found at 537
    Illegal BamHI site found at 1974
    Illegal BamHI site found at 3622
    Illegal XhoI site found at 575
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 2569
    Illegal XbaI site found at 2330
    Illegal XbaI site found at 4311
    Illegal SpeI site found at 821
    Illegal SpeI site found at 2585
    Illegal PstI site found at 1913
    Illegal PstI site found at 3719
    Illegal PstI site found at 3885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 2569
    Illegal XbaI site found at 2330
    Illegal XbaI site found at 4311
    Illegal SpeI site found at 821
    Illegal SpeI site found at 2585
    Illegal PstI site found at 1913
    Illegal PstI site found at 3719
    Illegal PstI site found at 3885
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 598
    Illegal SapI.rc site found at 1427


Design Notes

The DNA sequence was codon optimized for gene synthesis and for the expression in mammalian cells. The sequence for NT3 was attached to the C-terminus of the sequence for HIRMAb.


Source

This part was engineered through PCR and Gibson clonings.


References