![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4482014:Design
Designed by: Mustafo Mustafokulov Group: iGEM22_NYC_Empire_State (2022-10-10)
Human insulin receptor monoclonal antibody (HIRMAb)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3501
Illegal SpeI site found at 821
Illegal PstI site found at 2910
Illegal PstI site found at 3076 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 902
Illegal SpeI site found at 821
Illegal PstI site found at 2910
Illegal PstI site found at 3076
Illegal NotI site found at 3490 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 537
Illegal BamHI site found at 2813
Illegal XhoI site found at 575 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3501
Illegal SpeI site found at 821
Illegal PstI site found at 2910
Illegal PstI site found at 3076 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3501
Illegal SpeI site found at 821
Illegal PstI site found at 2910
Illegal PstI site found at 3076
Illegal AgeI site found at 1006 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1516
Illegal BsaI.rc site found at 598
Illegal SapI.rc site found at 1864
Design Notes
The DNA sequence was codon optimized for gene synthesis and for expression in mammalian cells.
Source
This part was engineered through PCR and Gibson clonings.