Coding

Part:BBa_K4482014:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-10-10)


Human insulin receptor monoclonal antibody (HIRMAb)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3501
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 902
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
    Illegal NotI site found at 3490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 537
    Illegal BamHI site found at 2813
    Illegal XhoI site found at 575
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3501
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3501
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
    Illegal AgeI site found at 1006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1516
    Illegal BsaI.rc site found at 598
    Illegal SapI.rc site found at 1864


Design Notes

The DNA sequence was codon optimized for gene synthesis and for expression in mammalian cells.


Source

This part was engineered through PCR and Gibson clonings.

References