Composite

Part:BBa_K4482011:Design

Designed by: Matteo Da Ros   Group: iGEM22_NYC_Empire_State   (2022-09-30)


HIRMAb-HC-FGF2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 262
    Illegal PstI site found at 1516
    Illegal PstI site found at 1522
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 262
    Illegal PstI site found at 1516
    Illegal PstI site found at 1522
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1342
    Illegal BamHI site found at 1413
    Illegal XhoI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 262
    Illegal PstI site found at 1516
    Illegal PstI site found at 1522
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 262
    Illegal PstI site found at 1516
    Illegal PstI site found at 1522
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1077
    Illegal BsaI.rc site found at 39
    Illegal SapI.rc site found at 387


Design Notes

The coding sequence for FGF2 was obtained from NCBI database (NP_001348594) and it was codon optimized for its synthesis by Twist Bioscience. The protein sequence for HIRMAb-HC was obtained from US patent US-8858950-B2 and back translated to obtain a DNA coding sequence for the protein, this DNA coding sequence was codon optimized for synthesis by Twist Bioscience and for expression in mammalian cells.


Source

the part was generated by gene synthesis and combined by Gibson assembly into a mammalian expression vector.

References