Coding

Part:BBa_K4482010:Design

Designed by: Matteo Da Ros   Group: iGEM22_NYC_Empire_State   (2022-09-26)


NT3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 585
    Illegal EcoRI site found at 678
    Illegal XbaI site found at 439
    Illegal SpeI site found at 694
    Illegal PstI site found at 22
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 585
    Illegal EcoRI site found at 678
    Illegal SpeI site found at 694
    Illegal PstI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 585
    Illegal EcoRI site found at 678
    Illegal BamHI site found at 83
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 585
    Illegal EcoRI site found at 678
    Illegal XbaI site found at 439
    Illegal SpeI site found at 694
    Illegal PstI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 585
    Illegal EcoRI site found at 678
    Illegal XbaI site found at 439
    Illegal SpeI site found at 694
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon optimized for synthesis.


Source

NCBI database for protein NT3: NP_001096124.

References