Coding

Part:BBa_K4482007:Design

Designed by: Matteo Da Ros   Group: iGEM22_NYC_Empire_State   (2022-09-26)


human insulin receptor light chain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 75
    Illegal PstI site found at 241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 75
    Illegal PstI site found at 241
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 75
    Illegal PstI site found at 241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 75
    Illegal PstI site found at 241
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA sequence was codon optimized for synthesis and for expression in mammalian cells. Kozan sequence was added around the start codon and SV40 polyA signal was added after the stop codon.


Source

The sequence was derived from the protein sequence registered in US patent US-8858950-B2.

References