Coding

Part:BBa_K4482006:Design

Designed by: Matteo Da Ros   Group: iGEM22_NYC_Empire_State   (2022-09-26)


human insulin receptor antibody heavy chain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 262
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1077
    Illegal BsaI.rc site found at 39
    Illegal SapI.rc site found at 387


Design Notes

The DNA sequence was codon optimized for gene synthesis and for the expression in mammalian cells. The bGH polyA signal was added at the end of the coding sequence for the HIRMAb heavy chain


Source

The sequence was derived from the protein sequence registered in US patent US-8858950-B2.

References