Coding

Part:BBa_K4482004:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-08-25)


FGFR2 eGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2276
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2276
    Illegal NheI site found at 2470
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2276
    Illegal BglII site found at 2084
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2276
    Illegal NgoMIV site found at 786
    Illegal NgoMIV site found at 1236
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1386
    Illegal BsaI.rc site found at 2238
    Illegal BsaI.rc site found at 3113
    Illegal SapI.rc site found at 106


Design Notes

The coding sequences were obtained by PCR with primers designed for Gibson assembly.


Source

These parts were obtained by PCR from plasmids obtained from AddGene.

References