![](https://parts.igem.org/images/partbypart/icon_rbs.png)
RBS
Part:BBa_K4477010:Design
Designed by: Miranda Khoury Group: iGEM22_Virginia (2022-09-06)
RBS for camelid anti-mouse VHH expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used a pre-existing RBS, BBa_J61100, as a starting point for optimization with Salis. We aimed for 75% relative expression so as to achieve a high level of protein expression of BBa_K4477005 without overburdening the E. coli expressing the gene. We optimized the RBS sequence to exclude restriction sites incompatible with various assembly methods.
Source
BBa_J61100 https://salislab.net/software/