Primer

Part:BBa_K4469013

Designed by: Leung Sum Yin Valerie   Group: iGEM22_CUHK-HongKong-SBS   (2022-09-30)


Adeno-associated virus serotype 2 (AAV2) inverted terminal repeats (ITR) FP

Universal forward primer used in AAV titration to determine transfer-plasmid containing AAV particles with real-time PCR (qPCR) techonolgy.

The ITRs of AAV2 consist of 145 nucleotides that forms a secondary hairpin structure. The primers (forward and reverse) were designed to target a 62-nucleotide seuqnece within the stem region of the ITR region.

The primers could be used for all AAV2 genome-based vector, which make titration of AAV2 simple and low cost.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None