Coding

Part:BBa_K4467002:Design

Designed by: Eloise Paine   Group: iGEM22_UNSW_Australia   (2022-10-10)


Pst_12806


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation was performed for expression of this part in NEB TURBO E.coli and NEB T7 Express E. coli.

Source

Pst_12806 encodes for a haustorium-specific protein from the wheat stripe rust fungus Puccinia striiformis f.sp.tritici (Pst). The genomic sequence was sourced from Xu, Q., et al. (2019).

References

XU, Q., et al. (2019). "An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function." Nature Communications 10(1): 5571.