Measurement

Part:BBa_K4462124

Designed by: Kredai Raaman   Group: iGEM22_IISc-Bengaluru   (2022-09-30)


pPfnr-sfGFP

Construct for hypoxic promoter characterisation: Pfnr-T7RBS-sfGFP-6xHis-Lt0_Ter

Usage and Biology

We used this part as a reference to measure the efficacy and regulation of the hypoxic promoters we wished to characterise.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal BamHI site found at 784
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None