Coding

Part:BBa_K4462053:Design

Designed by: Kredai Raaman   Group: iGEM22_IISc-Bengaluru   (2022-09-30)


TodB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 854
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1220


Design Notes

Source

Pseudomonas putida F1

References

Zylstra GJ, Gibson DT. Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli. J Biol Chem. 1989 Sep 5;264(25):14940-6. PMID: 2670929.