Coding

Part:BBa_K4462053

Designed by: Kredai Raaman   Group: iGEM22_IISc-Bengaluru   (2022-09-30)


TodB

FAD-dependent pyridine nucleotide-disulfide oxidoreductase from the Toluene dioxygenase operon of Pseudomonas putida F1, codon-optimised to reduce GC content

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 854
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1220


[edit]
Categories
Parameters
None