Part:BBa_K4462022

PanrEM7

Synthetic PEM7-derived promoter with the consensus ANR binding box from Pseudomonas putida KT2440 (Ugidos et al., 2008)

Usage and Biology

Transcriptional regulation with respect to oxygen availability in Pseudomonas species is principally achieved through the ANR (anaerobic regulation of arginine deaminase and nitrate reduction) global regulator. The dimeric active form of ANR carries a [4Fe–4S]²⁺ cluster and binds to the conserved ANR-binding box (5′-TTGATNNNNATCAA-3′) that is typically located in the -40 region of regulated promoters. Upon exposure to oxygen, this iron-sulphur cluster is partly destroyed and the ANR loses its DNA binding and gene regulatory ability. Depending on the position of its binding site, ANR can activate or repress transcription under hypoxic conditions where it exists in its active form.

This part is a modified version of the strong constitutive EM7 promoter (BBa_K4278705) that contains an ANR-binding box at the -40.5 position; we wished to determine if this is sufficient for induction by ANR under hypoxic conditions. We intended to use a Pseudomonas-compatible hypoxic promoter in our project to restrict the expression of Cytochrome P450cam to hypoxic conditions to enhance halocarbon biodegradation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]