Coding
Part:BBa_K4461013:Design
Designed by: SHIH-HSUN, YANG Group: iGEM22_NYCU-Taipei (2022-10-10)
mCherry+tag fusion protein
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 694
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 694
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 694
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 694
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 694
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We synthesized a commercial tag with EcoRI and HindIII cutting sites.
Source
gene synthesis