Regulatory

Part:BBa_K4458014:Design

Designed by: Maya Balakumaran   Group: iGEM22_East_Coast_BioCrew   (2022-10-01)


Cytochrome P450 Family 1 Subfamily A Member 1(CYP1A1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 3411
    Illegal PstI site found at 944
    Illegal PstI site found at 1021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2620
    Illegal PstI site found at 944
    Illegal PstI site found at 1021
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BglII site found at 721
    Illegal BglII site found at 1111
    Illegal BamHI site found at 697
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 3411
    Illegal PstI site found at 944
    Illegal PstI site found at 1021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 3411
    Illegal PstI site found at 944
    Illegal PstI site found at 1021
    Illegal NgoMIV site found at 2634
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1844
    Illegal BsaI.rc site found at 266


Design Notes

Due to high and low GC content, we had to modify a specific part of the sequence. We used Twist's codon optimization tool to reduce GC content. We tried to conserve regulatory regions in the fragment.


Source

NG_061374 Homo sapiens cytochrome P450 family 1 subfamily A member 1(CYP1A1), RefSeqGene (LRG_1262) on chromosome 15.

https://www.ncbi.nlm.nih.gov/nucleotide/NG_061374.1?report=genbank&log$=nuclalign&blast_rank=2&RID=KGYXABHP01R

References