Composite

Part:BBa_K4458004:Design

Designed by: Maxwell Swann   Group: iGEM22_East_Coast_BioCrew   (2022-09-10)


pcbA1 assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
    Illegal PstI site found at 131
    Illegal NotI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 700
    Illegal XhoI site found at 805
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
    Illegal NgoMIV site found at 1342
    Illegal AgeI site found at 1352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 343
    Illegal BsaI.rc site found at 415


Design Notes

Codon optimization for Rhodococcus jostii RHA1, full gene assembly


Source

Dehalococcoides mccartyi assembly

References