![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4458004:Design
Designed by: Maxwell Swann Group: iGEM22_East_Coast_BioCrew (2022-09-10)
pcbA1 assembly
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 13
Illegal XbaI site found at 234
Illegal PstI site found at 1
Illegal PstI site found at 131 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1
Illegal PstI site found at 131
Illegal NotI site found at 19 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 700
Illegal XhoI site found at 805 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 13
Illegal XbaI site found at 234
Illegal PstI site found at 1
Illegal PstI site found at 131 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 13
Illegal XbaI site found at 234
Illegal PstI site found at 1
Illegal PstI site found at 131
Illegal NgoMIV site found at 1342
Illegal AgeI site found at 1352 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 343
Illegal BsaI.rc site found at 415
Design Notes
Codon optimization for Rhodococcus jostii RHA1, full gene assembly
Source
Dehalococcoides mccartyi assembly