Coding

Part:BBa_K4458000:Design

Designed by: Rebecca Showalter   Group: iGEM22_East_Coast_BioCrew   (2022-09-10)


PCBA5 from Dehalococcoides mccartyi codon optimized for rhodococcus jostii rh1.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 766
    Illegal AgeI site found at 1193
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1156


Design Notes

Codon optimized for Rhodoccocus.

Source

Halococcoides mccartyi genome

References