Plasmid
Part:BBa_K4455005:Design
Designed by: Gabriella Cerna Group: iGEM22_ASU (2022-10-11)
FMDV 2A Peptide Optimized for Chlamydomonas Reinhardtii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 19
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 859
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This 2A peptide was codon optimized for usage in Chlamydomonas reinhardtii.
Source
The sequence for FMDV originates from Foot and Mouth Disease Virus. While the sequence for the 2A peptide itself originates from a virus, it only functions to enable the production of independent proteins when inserted between the coding sequences of proteins of interest.