Protein_Domain

Part:BBa_K4455003:Design

Designed by: Gabriella Cerna   Group: iGEM22_ASU   (2022-10-10)


IFV 2A Peptide Optimized for Chlamydomonas Reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This 2A peptide was codon optimized for usage in Chlamydomonas reinhardtii.


Source

The sequence for IFV originates from Infectious Flacherie Virus. While the sequence for the 2A peptide itself originates from a virus, it only functions to enable the production of independent proteins when inserted between the coding sequences of proteins of interest.

References