Reporter

Part:BBa_K4451022:Design

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


MutaT7 Test Cassette ATA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1599
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1599
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 850
    Illegal XhoI site found at 1731
    Illegal XhoI site found at 1937
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1599
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1599
    Illegal AgeI site found at 561
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was intended to test the in vivo function of the adenosine deaminase-T7 RNA polymerase fusion protein BBa_K4451004.

For more information on the rationale behind the design of this part, please refer to the part description of BBa_K4451021.

Source

References