DNA
GFA1

Part:BBa_K4450004:Design

Designed by: Andreas Sagen   Group: iGEM22_UiOslo_Norway   (2022-06-29)


Glutamine--fructose-6-phosphate aminotransferase [isomerizing] from Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 973
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 307
    Illegal BglII site found at 970
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 973
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 973
    Illegal AgeI site found at 112
    Illegal AgeI site found at 852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1184

Design Notes

Primers for isolation of the gene.
fwd: 5'-AACAATTTCACACAGGtttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagATGTGTGGTATCTTTGGTTACTG-3'
rev: 5'-GTAAGCTCATctagtatttctcctctttTTATTCGACGGTAACAGATTTAGC-3'

Source

Saccharomyces Cerevisiae ATCC 201388

References