Coding

Part:BBa_K4440000:Design

Designed by: Viacheslav Kiselev   Group: iGEM22_Estonia_TUIT   (2022-10-08)


VP2C-Tyr1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein should have been in one open reading frame without shift and the tyrosinase sequence was optimized for S. cerevisiae. Also illegal restriction sites for Biobrick compatability were removed


Source

Bacillus Megaterium (Xiaolin Shen et. al, 2019, DOI: 10.1038/s41598-019-43577-w) - Tyr1, Murine polyomavirus (Li Chen Cheah et. al., 2021, DOI: 10.1021/acssynbio.1c00045) - VP2C

References