Part:BBa_K4438602:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4438602

The graph depicts fluorescence readout of T6-D1 and T6-D2 after 30 mins and 1 hour incubation respectively.Dye: DFHBI dye + buffer; T6LA1: DFHBI dye + buffer + Trigger1-T6 Aptamer + Testosterone; T6LA2: DFHBI dye + buffer +Trigger2-T6 Aptamer + Testosterone

T6-Design 1: The fluorescence readout (Fig 2) indicates that the fold change in the fluorescence is not significant. Hence, there is no significant detection of testosterone. T6-Design 2: The fluorescence readout (Fig 2) indicates that the fold change in the fluorescence is significant of 4 folds for 1mM of testosterone. This result shows that T6-D3

After 30 minutes of incubation with 5X sensor buffer and dye, there was a shift in fluorescence intensity for 1mM testosterone compared to lower testosterone concentrations. An maximum fold-change of 4.23 was observed in the sample with 1mM testosterone, while the range of fold-change at lower concentrations was 1.15 to 1.34 folds.

This experiment paved the way for further optimization of testosterone-sensing dual aptamer systems. Our designs proved successful in detecting the hormone, however, further experiments could help us achieve a more sensitive system.

User Reviews

UNIQcbcb5a061ee370e8-partinfo-00000000-QINU UNIQcbcb5a061ee370e8-partinfo-00000001-QINU