DNA

Part:BBa_K4438161:Experience

Designed by: Diya Raj N   Group: iGEM22_IISER-Tirupati_India   (2022-10-12)


Applications of BBa_K4438161

T--IISER-Tirupati India--Fluroscence miR-222 (30 min).png

T--IISER-Tirupati India--Fluroscence miR 222 (1 hr).png Depicting fluorescence readout of FASTmiR-222-D2 after 30 mins and 1 hour incubation respectively.Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA

Explanation: After 30mins of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-222 sensor was increased in concentration. An average fold-change of 2.69 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 6.048 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.


After 1 hour, an average fold-change of 3.07 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 7.423 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.

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