DNA

Part:BBa_K4438121:Design

Designed by: Neha Adarsh   Group: iGEM22_IISER-Tirupati_India   (2022-10-11)


FASTmiR-222-D2_s


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 100
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 176
    Illegal SpeI site found at 100
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 100
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 100
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The split position of the complementary sequence in the sensor is ACCCAGTAGCC....AGAGTGAGCT



Source

Spinach aptamer

References