Coding

Part:BBa_K4437504:Design

Designed by: Marian Grell   Group: iGEM22_Calgary   (2022-10-12)


B0035-FLAG-phasin-HlyA (E. coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was designed as a test for experiments comparing RBS strength and resulting FLAG-phasin-HlyA expression levels, with the purpose of improving the expression system to increase phasin yields. As such, this part was designed with a weaker RBS than the original BBa_K2260002, to expand upon the relative expression levels of the other variable RBS types when combined with the FLAG-phasin-HlyA sequence.


Source

This part is based on the BBa_K2260002 part from the University of Calgary's 2017 iGEM team. The phasin (PhaP) gene is from R. eutropha, the HlyA tag is from E. coli, and the T7 promoter is from the T7 bacteriophage.

References