Composite

Part:BBa_K4432101:Design

Designed by: Paul Weimer   Group: iGEM22_Evry_Paris-Saclay   (2022-09-26)


PANTR1 Toehold Switch Sensor 01 with sfGFP-LVAtag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter (BBa_K2150031) + PANTR1 Toehold Switch Sensor 01 (BBa_K4432001) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong SBa_000587 Terminator (BBa_K3453000)

Source

DNA synthesis & Assembly

References