Coding

Part:BBa_K4429008:Design

Designed by: Anish Mulchandani   Group: iGEM22_IISER-Pune2_India   (2022-07-22)


scFv C10: EDE1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C10 scFv protein sequence obtained from PDB 7CTH [1], reverse translated and codon optimised for E. coli K12 using SnapGene

Removed BioBrick illegal restriction sites using silent mutation in SnapGene

Source

PDB 7CTH [1] -

References

[1] https://www.rcsb.org/structure/7CTH