Plasmid

Part:BBa_K4427021:Design

Designed by: Chanjuan Jin   Group: iGEM22_JLU_China   (2022-10-02)


pSB1C3-stuffer-LP2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 6035
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 2917
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 6036
    Illegal PstI site found at 2917
    Illegal PstI site found at 6050
    Illegal NotI site found at 7
    Illegal NotI site found at 6043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1165
    Illegal BamHI site found at 5958
    Illegal XhoI site found at 1743
    Illegal XhoI site found at 5967
    Illegal XhoI site found at 7067
    Illegal XhoI site found at 7959
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 6036
    Illegal PstI site found at 2917
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 6036
    Illegal PstI site found at 2917
    Illegal PstI site found at 6050
    Illegal NgoMIV site found at 1313
    Illegal AgeI site found at 1000
    Illegal AgeI site found at 3084
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

lp


Source

JLU_China

References