![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4425013
Designed by: Junfei Xia Group: iGEM22_SUSTech_Shenzhen (2022-09-30)
pET28a-BGL
We construct plasmid pET28a-BGL to express BGL to convert indican to indoxyl.
Usage and Biology
BGL was assemble with pET28a backbone to construct pET28a-BGL. BGL is induced to express to convert soluble indican to indoxyl, and the lattet can turn to indigo (or tyrian purple) by spontaneous oxidation
Source
BGL comes from P. tinctorium
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal XbaI site found at 47 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal NheI site found at 151 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal BamHI site found at 184
Illegal XhoI site found at 1618 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal XbaI site found at 47 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal XbaI site found at 47
Illegal AgeI site found at 529 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |