Composite

Part:BBa_K4425013

Designed by: Junfei Xia   Group: iGEM22_SUSTech_Shenzhen   (2022-09-30)


pET28a-BGL

We construct plasmid pET28a-BGL to express BGL to convert indican to indoxyl.


Usage and Biology

BGL was assemble with pET28a backbone to construct pET28a-BGL. BGL is induced to express to convert soluble indican to indoxyl, and the lattet can turn to indigo (or tyrian purple) by spontaneous oxidation

Source

BGL comes from P. tinctorium

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal NheI site found at 151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal BamHI site found at 184
    Illegal XhoI site found at 1618
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal XbaI site found at 47
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


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