Composite

Part:BBa_K4425009

Designed by: Junfei Xia   Group: iGEM22_SUSTech_Shenzhen   (2022-09-30)


pNEG

Express Taq polymerase when co-transform with trp repressor that cannot bind to its ligand.

Usage and Biology

Inactive trp repressors or trp repressor without its ligand can induce pNEG to express Taq polymerase. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 93
    Illegal XbaI site found at 85
    Illegal PstI site found at 1690
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 93
    Illegal NheI site found at 2136
    Illegal PstI site found at 1690
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 93
    Illegal BglII site found at 82
    Illegal BamHI site found at 1871
    Illegal XhoI site found at 424
    Illegal XhoI site found at 1501
    Illegal XhoI site found at 2458
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 93
    Illegal XbaI site found at 85
    Illegal PstI site found at 1690
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 93
    Illegal XbaI site found at 85
    Illegal PstI site found at 1690
    Illegal NgoMIV site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2014
    Illegal BsaI.rc site found at 2254
    Illegal SapI site found at 1326
    Illegal SapI site found at 2394
    Illegal SapI.rc site found at 1716
    Illegal SapI.rc site found at 2304


Reference

Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.

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