Composite
Part:BBa_K4425008
Designed by: Junfei Xia Group: iGEM22_SUSTech_Shenzhen (2022-09-30)
lacUV5-TrpR1
Express muted trp repressor when induced by IPTG
Usage and Biology
pTrpR is induced to express muted trp repressors. Active trp repressors with its ligand can induce pPOS to express Taq polymerase and inactive trp repressors or trp repressor without its ligand can induce pNEG to express Taq polymerase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 192
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 19
Illegal SapI.rc site found at 512
Reference
Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.
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Categories
Parameters
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