Regulatory

Part:BBa_K4425005

Designed by: Junfei Xia   Group: iGEM22_SUSTech_Shenzhen   (2022-09-30)


Trp operator

trp operator for trpR1 （BBa_K4425000）


Usage and Biology

a DNA sequence that can bind to TrpR1（BBa_K4425000） and repress the expression of downstream gene

Source

generate by Ellefson et al, 2018 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.

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