Coding

Part:BBa_K4425003

Designed by: Junfei Xia   Group: iGEM22_SUSTech_Shenzhen   (2022-09-30)


Taq polymerase

a DNA polymerase that can resist high temperature and perform PCR cycle


Usage and Biology

Taq polymerase was assembled into pNEG and pPOS to construct the circuits of positive and negative selection in trp repressor directed evolution.Positive clone in library can lead expression of taq polymerase. Induced Taq polymerase can performed a PCR cycle to replication the sequence of trp repressor

Figure 1: schematic of pNEG and pPOS

Source

Taq polymerase come from Thermus aquaticus

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2037
    Illegal PstI site found at 1591
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1772
    Illegal XhoI site found at 325
    Illegal XhoI site found at 1402
    Illegal XhoI site found at 2359
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1591
    Illegal NgoMIV site found at 1426
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1915
    Illegal BsaI.rc site found at 2155
    Illegal SapI site found at 1227
    Illegal SapI site found at 2295
    Illegal SapI.rc site found at 1617
    Illegal SapI.rc site found at 2205


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Categories
Parameters
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