Coding

Part:BBa_K4423001:Design

Designed by: Julia Vogl   Group: iGEM22_BOKU-Vienna   (2022-10-10)


Coding sequence for the main strain spider silk protein.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 227
    Illegal PstI site found at 422
    Illegal PstI site found at 434
    Illegal PstI site found at 581
    Illegal PstI site found at 776
    Illegal PstI site found at 788
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 227
    Illegal PstI site found at 422
    Illegal PstI site found at 434
    Illegal PstI site found at 581
    Illegal PstI site found at 776
    Illegal PstI site found at 788
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 227
    Illegal PstI site found at 422
    Illegal PstI site found at 434
    Illegal PstI site found at 581
    Illegal PstI site found at 776
    Illegal PstI site found at 788
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 227
    Illegal PstI site found at 422
    Illegal PstI site found at 434
    Illegal PstI site found at 581
    Illegal PstI site found at 776
    Illegal PstI site found at 788
    Illegal NgoMIV site found at 145
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence entails lots of repeating sections, which had to be considered during the codon optimization. Furthermore it is not possible to synthesize the whole sequence at once, it was devided in several parts that were connected through multiple PCR reactions.


Source

The sequence used was taken from "Bin Liu, Tao Wang, Liyan Xiao, Guilan Zhang, Guangshen Li, Jingzhi Luo & Xiaobing Liu (2018) A directed self-assembly quasi-spider silk protein expressed in Pichia pastoris, Biotechnology & Biotechnological Equipment, 32:2, 451-461, DOI: 10.1080/13102818.2017.1327823" and later codonoptimized using the codon optimization tool of the company IDT.

References