Generator

Part:BBa_K4419025:Design

Designed by: Leonardo ken Okumura, Ryoko Enomoto   Group: iGEM22_TokyoTech   (2022-10-11)


pCAG-loxP_NLS_NCre_IRES_GFP_loxP_mCherry (Human)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 894
    Illegal PstI site found at 1279
    Illegal PstI site found at 3109
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 894
    Illegal PstI site found at 1279
    Illegal PstI site found at 3109
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 894
    Illegal PstI site found at 1279
    Illegal PstI site found at 3109
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 894
    Illegal PstI site found at 1279
    Illegal PstI site found at 3109
    Illegal NgoMIV site found at 2674
    Illegal NgoMIV site found at 3428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2639


Design Notes

Human (Homo sapiens) codon optimization on proteins N-Cre and mCherry was performed by using VectorBuilder.


Source

N/A

References