Coding

Part:BBa_K4417025

Designed by: Jiaying Zou   Group: iGEM22_UCL   (2022-10-12)


ureABCEFG

Description

This part is the nature urease operon, which could be cloned into pCT5c. This basic ureABC and ureEFG gene could be cloned together using BBa_K4417016 and BBa_K4417024 using golden gate assembly. (more details can be found on BBa_K4417017).

This part was used to clone into master plasmid BBa_K4417017 using golden gate assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3403
    Illegal XhoI site found at 4157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None