Composite

Part:BBa_K4417021

Designed by: Jiaying Zou   Group: iGEM22_UCL   (2022-10-12)


CA22- rrnB T1 Terminator

Description

This part is a codon optimized sequence for an α-type carbonic anhydrase operon from Bacillus halodurans TSLV1. It contained E. coli. Terminator (BBa_K4417011) and was cloned into our pCT5c Type IIS compatible plasmid (BBa_K4417000).

Figure 1:CA composite part.

Method

This part can be cloned into pCT5c plasmid using NdeI and SacI sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 827
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None