Coding

Part:BBa_K4415001:Design

Designed by: William Pihlainen-Bleecker   Group: iGEM22_McMaster_A   (2022-09-29)


Anti-CD22 antibody scFv


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed for insertion into the f3-55nm-MCS phage vector for display on the pIII coat protein. The protein coding sequence is bound by 2 SfiI restriction enzyme sites, with the stop codon removed to allow for continuous synthesis of the pIII protein. 2 addition base pairs were added after the last codon, which puts the insert into frame

Source

Amino acid sequence came from GenBank - KM196172.1 Insertion designed based on https://www.nature.com/articles/nprot.2007.51

References