DNA

Part:BBa_K4414030

Designed by: Shiyin Jin   Group: iGEM22_NUDT_CHINA   (2022-09-22)


LBD-GSG-NES-GSG-EGFP

Validate that the glucocorticoid-stimulated entry from the cytoplasm into the nucleus can be accomplished by retaining only the LBD fragment and add the NES sequence to weaken the entry of the GR.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 745
    Illegal PstI site found at 289
    Illegal PstI site found at 851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 745
    Illegal PstI site found at 289
    Illegal PstI site found at 851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 745
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 745
    Illegal PstI site found at 289
    Illegal PstI site found at 851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 745
    Illegal PstI site found at 289
    Illegal PstI site found at 851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 543
    Illegal SapI.rc site found at 866


[edit]
Categories
Parameters
None