DNA

Part:BBa_K4409006:Design

Designed by: Ruyi Shi   Group: iGEM22_Worldshaper-HZ   (2022-09-20)


dsDNA (GAPDH)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A DNA sequence of GAPDH is used as the substrate target dsDNA region for the cleavage of the CRISPR-Cas system, because GAPDH gene is abundantly presented in cells and can be easily amplified. This dsDNA needs to be used with the guide sequence BBa_K4409017 in a gRNA. The dsDNA also contains a PAM site which is required for a Cas nuclease to cut. In the Cas12 protein, the sequence for the PAM site is TTTX, so we used TTTA.


Source

This part is based on the GAPDH gene sequence retrieved from Ensembl database.

References