Plasmid

Part:BBa_K4404010:Design

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)

acsB2 from C. ljungdahlii under the control of a PbgaL promoter from C. perfringens


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal XbaI site found at 6125
    Illegal SpeI site found at 1777
    Illegal PstI site found at 542
    Illegal PstI site found at 5988
    Illegal PstI site found at 6164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal NheI site found at 2230
    Illegal NheI site found at 6343
    Illegal SpeI site found at 1777
    Illegal PstI site found at 542
    Illegal PstI site found at 5988
    Illegal PstI site found at 6164
    Illegal NotI site found at 6067
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal BglII site found at 6152
    Illegal BamHI site found at 2214
    Illegal BamHI site found at 6119
    Illegal XhoI site found at 97
    Illegal XhoI site found at 6156
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal XbaI site found at 6125
    Illegal SpeI site found at 1777
    Illegal PstI site found at 542
    Illegal PstI site found at 5988
    Illegal PstI site found at 6164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal XbaI site found at 6125
    Illegal SpeI site found at 1777
    Illegal PstI site found at 542
    Illegal PstI site found at 5988
    Illegal PstI site found at 6164
    Illegal NgoMIV site found at 3537
    Illegal NgoMIV site found at 5444
    Illegal NgoMIV site found at 5454
    Illegal NgoMIV site found at 5580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5196
    Illegal SapI.rc site found at 5551
    Illegal SapI.rc site found at 5922


Design Notes

It is important to produce inserts with specific restriction enzymes in order to secure a robust implementation into a vector system.


Source

The constitutive promoter originated from C. perfringens, while acsB2 was amplified from genomic DNA of Clostridium ljungdahlii with specific primers by PCR. The pMTL83151 based on the ClosTron plasmid system and was modified by Prof. Peter Dürre (department of microbiology and biotechnology).

References