Coding

Part:BBa_K4404006:Design

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)


acsB, acsA and cooC2 from A. woodii with SfaA1 restriction site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 692
    Illegal EcoRI site found at 4268
    Illegal EcoRI site found at 4613
    Illegal EcoRI site found at 4901
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 692
    Illegal EcoRI site found at 4268
    Illegal EcoRI site found at 4613
    Illegal EcoRI site found at 4901
    Illegal NheI site found at 4955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 692
    Illegal EcoRI site found at 4268
    Illegal EcoRI site found at 4613
    Illegal EcoRI site found at 4901
    Illegal BamHI site found at 4939
    Illegal XhoI site found at 303
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 692
    Illegal EcoRI site found at 4268
    Illegal EcoRI site found at 4613
    Illegal EcoRI site found at 4901
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 692
    Illegal EcoRI site found at 4268
    Illegal EcoRI site found at 4613
    Illegal EcoRI site found at 4901
    Illegal AgeI site found at 2884
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2137


Design Notes

It is important to produce overhangs which flank the region for the digest with specific restriction enzymes in order to secure a robust implementation and combination of two inserts into one vector system.


Source

Ordered by IDT with the genomic background of Acetobacerium woodii.

References