Composite

Part:BBa_K4399008:Design

Designed by: Duoqing LIN   Group: iGEM22_Worldshaper-Shanghai   (2022-09-27)


PAtUBI5- GFP-Tmas


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1342
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1342
    Illegal NheI site found at 1244
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1342
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1342
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1342
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Transcription of GFP (BBa_K4399002) is initiated by PAtUBI5 (BBa_K4399000) and terminated byTmas (BBa_K4399005).


Source

The sequence is based on BBa_K4399000, BBa_K4399002 and BBa_K4399005.

References