Coding
Part:BBa_K4398005:Design
Designed by: Reinis Muiznieks Group: iGEM22_Latvia-Riga (2022-10-11)
Hygromycin resistance for Rhodotorula toruloides
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 104
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 904
Illegal AgeI site found at 342 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
While performing codon optimization the high GC content prevented custom DNA synthesis to be performed. Therefore, a balance between the use of the most frequently used codons and minimization of the GC content was found. This was done by limiting the GC content to <60% in DNA Chisel.
Source
The sequence which was optimized is taken from https://www.addgene.org/35122/sequences/