Coding

Part:BBa_K4398005:Design

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


Hygromycin resistance for Rhodotorula toruloides


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 904
    Illegal AgeI site found at 342
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While performing codon optimization the high GC content prevented custom DNA synthesis to be performed. Therefore, a balance between the use of the most frequently used codons and minimization of the GC content was found. This was done by limiting the GC content to <60% in DNA Chisel.


Source

The sequence which was optimized is taken from https://www.addgene.org/35122/sequences/

References